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DNeasy Blood & Tissue Kit
用于从动物的血液和组织，细胞，酵母，细菌和病毒中提取总DNA
- 重复性好 — 为各种样本提供标准化的纯化方法
- 即使是特殊类型起始样本也可以获得高产量DNA
- 高品质DNA — 用于敏感的下游应用， 如多重及定量 PCR
- 经过优化的操作方法适合处理各种起始样本
- 可满足多种通量的需要 — 有离心柱方式或96孔板高通量方式
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DNeasy Blood & Tissue Kits provide fast and easy silica-based DNA purification in convenient spin-column and 96-well-plate formats. Most samples can be directly lysed with proteinase K, eliminating the need for mechanical disruption and reducing hands-on time. Optimized protocols for specific sample types provide reproducible purification of high-quality DNA for life science, genotyping, and veterinary pathogen research applications. Purification of DNA using the DNeasy Blood & Tissue Kit can be automated on the QIAcube.
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DNeasy 96 Blood & Tissue Kit (4) |
For 4 x 96 DNA minipreps: 4 DNeasy 96 Plates, Proteinase K, Buffers, S-Blocks, AirPore Tape Sheets, Collection Microtubes (1.2 ml), Elution Microtubes RS, Caps, 96-Well Plate Registers |
69581 |
Inquire |
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DNeasy 96 Blood & Tissue Kit (12) |
For 12 x 96 DNA minipreps: 12 DNeasy 96 Plates, Proteinase K, Buffers, S-Blocks, AirPore Tape Sheets, Collection Microtubes (1.2 ml), Elution Microtubes RS, Caps, 96-Well Plate Registers |
69582 |
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DNeasy Blood & Tissue Kit (250) |
250 DNeasy Mini Spin Columns, Proteinase K, Buffers, Collection Tubes (2 ml) |
69506 |
￥6,620.00 |
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DNeasy Blood & Tissue Kit (50) |
50 DNeasy Mini Spin Columns, Proteinase K, Buffers, Collection Tubes (2 ml) |
69504 |
￥1,540.00 |
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| RNeasy Mini Kit (50) |
从动物细胞或组织，酵母或细菌中，纯化多达100 ug 总RNA . Contents: 50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers |
74104 |
￥3,040.00 |
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| QIAquick PCR Purification Kit (50) |
纯化PCR产物，100bp-10kb. Contents: For purification of 50 PCR reactions: 50 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml) |
28104 |
￥1,110.00 |
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| RNase-Free DNase Set (50) |
用于RNA纯化过程中的DNA酶消化. Contents: 1500 units RNase-free DNase I, RNase-free Buffer RDD, and RNase-free water for 50 RNA minipreps |
79254 |
￥1,030.00 |
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| RNase A (17,500 U) |
用于DNA制备中RNA酶的消化. Contents: 2.5 ml (100 mg/ml; 7000 units/ml, solution) |
19101 |
￥1,750.00 |
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| QIAGEN Proteinase K (2 ml) |
用于DNA和RNA制备过程中蛋白酶的消化. Contents: 2 ml (>600 mAU/ml, solution) |
19131 |
￥830.00 |
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The DNeasy Blood & Tissue Kit is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention, or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.
Principle
DNeasy Blood & Tissue Kits simplify purification of DNA from a wide range of sample types, including animal species commonly encountered in life science, veterinary, and genotyping applications (see figure "High-Quality DNA from Various Animal Tissues").
The efficient DNeasy Blood & Tissue procedure enables high yields of total DNA from animal blood and tissue samples (see table "Typical DNA Yields from Animal Tissues Using DNeasy Blood & Tissue Kits" and figure "Typical DNA Yields from Animal Blood Using DNeasy Blood & Tissue Kits"). Optimized protocols ensure high yields from nonstandard samples, such as animal hair (see figure "Genotyping with DNA from Horse Hairs"), as well as cultured cells, fixed tissues, or Gram-positive and -negative bacteria. Specialized online protocols are available for DNA purification from yeast, insects, hair, and other sample types.
Procedure
Reliable silica-membrane technology, in convenient spin-column or 96-well formats, ensures fast and reproducible DNA purification, eliminating the need for organic extraction and alcohol precipitation (see flowchart "DNeasy Mini and 96 Procedures"). DNeasy Mini spin columns are prepackaged in collection tubes and individually sealed, providing convenience and safety. The DNeasy 96 Blood & Tissue Kit provides high-throughput processing in a 96-well format using the QIAGEN 96-Well-Plate Centrifugation System.
Applications
DNeasy Blood & Tissue Kits provide reliable results — from laboratory analysis of transgenic mice (see figure "High-Throughput DNA Purification Using the DNeasy 96 Blood & Tissue Kit") to livestock breeding programs (see figure "Genotyping of Pigs for Quality Management of Livestock") and pedigree genotyping. Routine testing applications can be easily scaled up using the DNeasy 96 Blood & Tissue Kit (see figure "High-Throughput DNA Purification Using the DNeasy 96 Blood & Tissue Kit").
Purified DNA is free from PCR inhibitors, enabling sensitive detection in standard, multiplex (see figure "Efficient 16plex PCR over a Range of Template Amounts"), and real-time PCR (see figure "Real-Time PCR Detection of Borrelia DNA in Ticks"). DNeasy Blood & Tissue Kits provide high-quality DNA, ready to use in all downstream assays, including applications in:
- Life science research
- Livestock breeding
- Pedigree genotyping
- Veterinary pathogen research
- Routine applied testing
Images
Typical DNA Yields from Animal Tissues Using DNeasy Blood & Tissue Kits
High-Quality DNA from Various Animal Tissues
Typical DNA Yields from Animal Blood Using DNeasy Blood & Tissue Kits
Genotyping with DNA from Horse Hairs
DNeasy Mini and 96 Procedures
High-Throughput DNA Purification Using the DNeasy 96 Blood & Tissue Kit
Genotyping of Pigs for Quality Management of Livestock
Efficient 16plex PCR over a Range of Template Amounts
Real-Time PCR Detection of Borrelia DNA in Ticks
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