Transfection Cell Database - Detailed View


Cell Line: AtT-20

Cell Line Species/Tissue: mouse / pituitary tumor 
Transfection Reagent: Effectene 
Nucleic Acid:
DNA 
Growth Medium:
DMEM + antibiotics + glutamin 
Percent Serum (%):
Reporter System:
gene of interest 
Plasmid Purification Method:
QIAfilter Plasmid Kit 
Plate Format:
100-mm dish 
Number of Cells:
 
Percent Confluence(%):
 
Amount of Nucleic Acid (µg):
Amount of Enhancer (µl):
16 
Amount of Reagent (µl):
60 
Complex Incubation on Cells (hrs):
Analysis Performed Post-Transfection (hrs):
 
Transfection Efficiency (%):
>80% 
 
Any modifications to the protocol?:
 
Notes:
 
References: