Transfection Cell Database - Detailed View


Cell Line: BHK-21

Cell Line Species/Tissue: hamster / normal kidney, fibroblast 
Transfection Reagent: SuperFect 
Nucleic Acid:
DNA 
Growth Medium:
DMEM 
Percent Serum (%):
10 
Reporter System:
b-gal 
Plasmid Purification Method:
QIAGEN Plasmid Kit 
Plate Format:
100-mm dish 
Number of Cells:
 
Percent Confluence(%):
 
Amount of Nucleic Acid (µg):
Amount of Enhancer (µl):
 
Amount of Reagent (µl):
10 
Complex Incubation on Cells (hrs):
 
Analysis Performed Post-Transfection (hrs):
 
Transfection Efficiency (%):
75% 
 
Any modifications to the protocol?:
 
Notes:
Efficiency determined by counting X-gal stained cells.  
References: