| Cell Line Species/Tissue: |
Mouse
/
Melanocyte |
| Transfection Reagent: |
SuperFect |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM+10%serum |
Percent Serum (%):
|
|
Reporter System:
|
GFP |
Plasmid Purification Method:
|
EndoFree Plasmid Kit |
Plate Format:
|
24-well plate |
Number of Cells:
|
|
Percent Confluence(%):
|
40 % |
Amount of Nucleic Acid (µg):
|
6 µg |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
30 µl |
Complex Incubation on Cells (hrs):
|
3 h |
Analysis Performed Post-Transfection (hrs):
|
18 h |
Transfection Efficiency (%):
|
50 % |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| |
| References: |
| |