| Cell Line Species/Tissue: |
Primary effusion leukemia
/
|
| Transfection Reagent: |
HiPerFect |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
RPMI 1640 |
Percent Serum (%):
|
105 |
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
6-well dishes |
Number of Cells:
|
1x10e5 cell |
Percent Confluence(%):
|
see reference |
Amount of Nucleic Acid (µg):
|
see reference |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
see reference |
Complex Incubation on Cells (hrs):
|
|
Analysis Performed Post-Transfection (hrs):
|
48h |
Transfection Efficiency (%):
|
|
| Knockdown Efficiency (%): |
80% |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| |
| References: |
| Regulation of Microfilament Organization by Kaposi Sarcoma-associated Herpes Virus-cyclin·CDK6 Phosphorylation of Caldesmon
Maria Emanuela Cuomo, Axel Knebel, Georgina Platt, Nick Morrice, Philip Cohen, and Sibylle Mittnacht
J. Biol. Chem., Oct 2005; 280: 35844 - 35858.
|