| Cell Line Species/Tissue: |
hepatocellular carcinoma
/
|
| Transfection Reagent: |
HiPerFect |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
10xFBS in DMEM |
Percent Serum (%):
|
10 % |
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
6-well |
Number of Cells:
|
|
Percent Confluence(%):
|
50% |
Amount of Nucleic Acid (µg):
|
1-100ng |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
6-24 µl |
Complex Incubation on Cells (hrs):
|
48 h |
Analysis Performed Post-Transfection (hrs):
|
48 h |
Transfection Efficiency (%):
|
|
| Knockdown Efficiency (%): |
97 % (RNA) 95 % (Protein) |
| |
| Any modifications to the protocol?:
|
| Yes, see notes |
| Notes: |
| Dish: collagen coated
Experiment with different concentrations of nufcleic acid( 1-100ng).
6hr after passage, change medium. And after further 6hr, transfected.
Analysis method: Real-Time PCR, Immunoblotting |
| References: |
| |