| Cell Line Species/Tissue: |
Hamster
/
|
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
|
Percent Serum (%):
|
10 |
Reporter System:
|
GFP |
Plasmid Purification Method:
|
QIAGEN Plasmid Kit |
Plate Format:
|
6-well plate |
Number of Cells:
|
|
Percent Confluence(%):
|
50-80 |
Amount of Nucleic Acid (µg):
|
0.8 |
Amount of Enhancer (µl):
|
10 |
Amount of Reagent (µl):
|
6.4 |
Complex Incubation on Cells (hrs):
|
6 |
Analysis Performed Post-Transfection (hrs):
|
24 |
Transfection Efficiency (%):
|
60 |
| |
| Any modifications to the protocol?:
|
| Used Effectene at a ratio of 12.5 ul per ug of DNA. |
| Notes: |
| |
| References: |
| |